QuantaBio/sparQ PureMag Beads/60 mL/95196-060

sparQ PureMag Beads is a fast and reliable nucleic acid purification system for reaction cleanup and size selection in Next Generation Sequencing (NGS) workflows. Based on the reversible nucleic acid-binding properties of magnetic beads, this product can be used to quickly remove primers, primer-dimers, unincorporated nucleotides, salts, adapters and adapter-dimers from NGS library prep reactions to improve downstream sequencing performance. sparQ PureMag Beads allows excellent recovery of fragments greater than 100 bp without centrifugation or filtration. Consistent and reliable size selection can be achieved by simply adjusting the beads to sample ratio. This product is designed for both manual and automated processing, allowing seamless integration into existing workflows.

Workflow for Double-Sided Size Selection

Performance Data

Consistent and tunable size selection

Bioanalyzer trace of fragmented human genomic DNA pre- and post double-sided size selection

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Bioanalyzer trace of fragmented human genomic DNA pre- and post double-sided size selection

Electropherogram of fragmented human genomic DNA pre- and post double-sided size selection. Different sparQ PureMag Beads to DNA ratios were used to achieve various targeted size range.

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Highly reproducible purification across a range of inputs

Highly reproducible purification across a range of inputs

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Highly reproducible purification across a range of inputs

Highly reproducible DNA library profiles were achieved using different lots of sparQ PureMag Beads and a broad range of input amount. Libraries were prepared with sparQ DNA Library Prep Kit from 100 ng and 1 ng of fragmented microbial genomic DNA. sparQ PureMag Beads were used post adapter ligation and PCR amplification to effectively remove adapter-dimers and primer-dimers.

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Efficient recovery of DNA equivalent to AMPure XP

Efficient recovery of DNA equivalent to AMPure XP

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Efficient recovery of DNA equivalent to AMPure XP

sparQ PureMag Beads show equivalent performance toAMPure XP for DNA purification. 50 bp DNA ladder was purified with sparQ PureMag Beads and AMPure XP at different beads to DNA ratios and analyzed on 2% agarose gel.

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